ABSTRACT
'Whole' cauda epididymal homogenate and individual components of the cauda epididymis viz, cauda epididymal cells, epididymal plasma and sperm cells, as well as the blood plasma of the rat were screened for renin-like activity by in vivo method. Enzyme activity was high in 'whole' cauda epididymal homogenate, but very low in blood plasma. Evaluation of individual components of the epididymis revealed a relatively high enzyme activity in the epididymal cells but low activity in epididymal plasma and epididymal sperm cells. The high activity in epididymal cells was not affected by efferent duct ligation for 8 weeks and bilateral nephrectomy. Like most other epididymal functions, renin-like activity in the epididymis was androgen dependent. It was concluded that cauda epididymis of the rat contains the enzyme renin, whose activities may be predominantly intracellular.
Subject(s)
Animals , Bile Ducts/physiology , Epididymis/cytology , Male , Nephrectomy , Orchiectomy , Rats , Rats, Sprague-Dawley , Renin/blood , Spermatozoa/enzymologySubject(s)
Humans , Male , Female , Child , Adolescent , Adult , Aged , Bile Ducts/anatomy & histology , Bile Ducts/physiology , Liver/anatomy & histology , Liver/physiology , Gastroenterology , Gastrointestinal Diseases/diagnosis , Intestine, Large/anatomy & histology , Intestine, Large/physiology , Pancreas/anatomy & histology , Pancreas/physiologyABSTRACT
A atividade eletromiográfica do esfíncter de Oddi e a pressäo biliar foram determinadas em sete opossums com vesícula biliar intacta e após a realizaçäo de colecistectomia. Morfina em dose de 0.2 mg/kg foi administrada endovenosamente após a obtençäo de registros de controle. O efeito de diferentes quantidades de infusäo de soluçäo salina sobre a pressäo biliar também foi estudado. Näo houve significantes alteraçöes na pressäo biliar entre os registros de controle (13.3 ñ 2.1 mmHg) e após a administraçäo de morfina (13.8 ñ 2.2 mmHg) nos opossums com vesícula biliar intacta. Entretanto, a pressäo biliar após a administraçäo de morfina (17.2 ñ 1.5 mmHg) foi significantemente mais elevada do que as pressöes de controle (13.2 ñ 2.3 mmHg) nos animais que tinham sido submetidos à colecistectomia (p < 0.01). Concluiu-se que a morfina näo aumenta a pressäo biliar em animais com vesícula biliar intacta, mas o faz em animais que foram submetidos à colecistectomia